Composite
Part:BBa_K887000:Design
Designed by: LIN I Group: iGEM12_NCTU_Formosa (2012-08-19)
Plac+B0034+zif268+alsS+B0034+PBSII+ilvC+B0034+HIVC+ilvD+37℃ induced RBS+tetR+double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 6049
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6938
Illegal AgeI site found at 3158
Illegal AgeI site found at 4143 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2744
Illegal BsaI site found at 5835
Illegal BsaI site found at 6092
Illegal BsaI.rc site found at 836
Illegal BsaI.rc site found at 1430
Illegal BsaI.rc site found at 3563
Design Notes
The first part of this complete biobrick is built in Plac, two strong and one weak expressing RBS, Alss, ilvC, ilvD, 37°C regulator RBS, tetR and double terminator. Before the enzymes,there are zic fingers from 2010 Slovenia iGEM team. And we also corrected the missing base pair of DNA program from 2010 Slovenia iGEM team.
Source
It is improved form NCTU-Formosa 2011 iGEM team.